Anti‐diabetic effect of andrographolide from Sambiloto herbs (Andrographis paniculata (Burm.f.) Nees) through the expression of PPARγ and GLUT‐4 in adipocytes
نویسندگان
چکیده
Andrographolide has been shown to have a pharmacological effect as an antidiabetic. Nevertheless, the comprehensive mechanism of action yet be determined. is primary component sambiloto herb (Andrographis paniculata (Burm.f.) Nees), in which simple isolation process can obtain high yields. This study aimed explain anti‐diabetic andrographolide compared pioglitazone (a positive control) on glucose uptake by measuring expression levels peroxisome proliferator‐activated receptor gamma (PPARγ) and transporter type 4 (GLUT‐4) genes 3T3‐LI mouse adipocytes vitro model. The differentiation mature from 3T3‐L1 fibroblasts was induced with 3‐isobutyl‐1‐methylxanthine, dexamethasone, insulin. provided through direct A. herbs. gene detected using reverse transcription‐polymerase chain reaction (RT‐PCR). Pioglitazone significantly increased capability. able increase mRNA PPARγ GLUT‐4 best concentration at 5.6 µM. In conclusion, improve increasing that encodes protein, are key factors for homeostasis. Therefore, this finding further establishes potency
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ژورنال
عنوان ژورنال: Indonesian Journal of Biotechnology
سال: 2022
ISSN: ['0853-8654', '2089-2241']
DOI: https://doi.org/10.22146/ijbiotech.68800